In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

InhaltsangabePart I: Metabolites and Metabolic Fluxes1. MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary MetabolismCatherine Deborde and Daniel Jacob 2. Targeted Deuteration of Polyphenolics for their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived ExtractsMikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman 3. Relative Quantitation in Single Cell Metabolomics by Laser Ablation Electrospray Mass SpectrometryBindesh Shrestha and Akos Vertes 4. Quantification of Plant VolatilesAnthony V. Qualley and Natalia Dudareva 5. Quantitative Imaging Approaches for Small Molecule Measurements using FRET Sensors in PlantsSakiko Okumoto 6. Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic ResonanceQuyen X. Truong, Jong Moon Yoon, and  Jacqueline V. Shanks 7. Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass SpectrometryJamey D. Young, Douglas K. Allen, and John A. Morgan 8. Mathematical Modeling of Isotope Labeling Experiments for Metabolic Flux AnalysisShilpa Nargund and Ganesh Sriram 9. Optimal Design of Isotope Labeling ExperimentsHong Yang, Dominic E Mandy and Igor GL Libourel Part II: Metabolic Pathways and Metabolic Systems Biology10. Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New CapabilitiesKate Dreher 11. Elucidation of Metabolic Pathways from Enzyme Classification DataAndrew G. McDonald and Keith F. Tipton  12. Deducing Intracellular Distributions of Metabolic Pathways from Genomic DataAnsgar Gruber and Peter G. Kroth 13. Genome-scale Models of Plant MetabolismMargaret Simons, Ashish Misra, and Ganesh Sriram 14. Elementary Flux Modes, Flux Balance Analysis and their Application to Plant MetabolismKatrin Lotz, Anja Hartmann, Eva Grafahrend-Belau, Falk Schreiber, and Björn H. Junker 15. Systems Approaches to Unraveling Plant Metabolism: Identifying Biosynthetic Genes of Secondary Metabolic Pathways Martin J. Spiering, Bhavneet Kaur, James F. Parsons, and Edward Eisenstein  16. Applications of Kinetic Modeling toPlant MetabolismJohann M. Rohwer 17. Kinetic Modeling of Plant Metabolism and its Predictive Power - Peppermint Essential Oil Biosynthesis as an ExampleBernd Markus Lange and Rigoberto Rios-Estepa