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Genomic Imprinting

Methods and Protocols, Methods in Molecular Biology 925

Erschienen am 21.08.2012, 1. Auflage 2012
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Bibliografische Daten
ISBN/EAN: 9781627030106
Sprache: Englisch
Umfang: xi, 297 S.
Einband: gebundenes Buch

Beschreibung

InhaltsangabePart I: Parent-of-Origin Effects 1. Uniparental Embryos in the Study of Genomic Imprinting            Yong Cheng, Dasari Amarnath, and Keith E. Latham 2. Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species            Masanori Imamura, Hironobu Okuno, Ikuo Tomioka, Yoshimi Kawamura, Zachary Yu-Ching Lin, Ryusuke Nakajima, Wado Akamatsu, Hirotaka James Okano, Yumi Matsuzaki, Erika Sasaki, and Hideyuki Okano 3. Generation of Trophoblast Stem Cells            Michael C. Golding 4. Immunomagnetic Purification of Murine Primordial Germ Cells            Emily Y. Smith and James L. Resnick Part II: Identifying Imprinted Genes 5. Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA            Andrew J. Sharp 6. Identification of Imprinted Loci by Transcriptome Sequencing            Tomas Babak 7. Data Mining as a Discovery Tool for Imprinted Genes            Chelsea Brideau and Paul Soloway Part III: Identifying the Regulatory Features of Imprinted Domains 8. Engineering of Large Deletions and Duplications In Vivo            Louis Lefebvre Part IV: Epigenetics of Imprinted Regions 9. Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells            Julie Borgel, Sylvain Guibert, and Michael Weber 10. Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains            Purnima Singh and Piroska E. Szabó 11. Quantitative Chromosome Conformation Capture            Raffaella Nativio, Yoko Ito, and Adele Murrell 12. Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing            Sébastien A. Smallwood and Gavin Kelsey Part V: Analysis of Imprinted Expression 13. Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies            Sarah Rose Huffman, Md Almamun, and Rocío Melissa Rivera 14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs            Mathieu Rederstorff 15. Co-Immunoprecipitation of Long Non-Coding RNAs            Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil Part VI: Imprinting in Plants 16. Specialized Technologies for Epigenetics in Plants            Wenyan Xiao Part VII: Evolution of Imprinted Genes 17. Computational Studies of Imprinted Genes            Martina Paulsen 18. Insights on Imprinting from Beyond Mice and Men            Andrew Pask 19. Non-Mammalian Parent-Of-Origin Effects            Elena de la Casa-Esperón 14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs            Mathieu Rederstorff 15. Co-Immunoprecipitation of Long Non-Coding RNAs            Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil Part VI: Imprinting in Plants 16. Specialized Technologies for Epigenetics in Plants            Wenyan Xiao Part VII: Evolution of Imprinted Genes 17. Computational Studies of Imprinted Genes            Martina Paul

Autorenportrait

InhaltsangabePart I: Parent-of-Origin Effects 1. Uniparental Embryos in the Study of Genomic Imprinting            Yong Cheng, Dasari Amarnath, and Keith E. Latham 2. Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species            Masanori Imamura, Hironobu Okuno, Ikuo Tomioka, Yoshimi Kawamura, Zachary Yu-Ching Lin, Ryusuke Nakajima, Wado Akamatsu, Hirotaka James Okano, Yumi Matsuzaki, Erika Sasaki, and Hideyuki Okano 3. Generation of Trophoblast Stem Cells            Michael C. Golding 4. Immunomagnetic Purification of Murine Primordial Germ Cells            Emily Y. Smith and James L. Resnick Part II: Identifying Imprinted Genes 5. Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA            Andrew J. Sharp 6. Identification of Imprinted Loci by Transcriptome Sequencing            Tomas Babak 7. Data Mining as a Discovery Tool for Imprinted Genes            Chelsea Brideau and Paul Soloway Part III: Identifying the Regulatory Features of Imprinted Domains 8. Engineering of Large Deletions and Duplications In Vivo            Louis Lefebvre Part IV: Epigenetics of Imprinted Regions 9. Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells            Julie Borgel, Sylvain Guibert, and Michael Weber 10. Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains            Purnima Singh and Piroska E. Szabó 11. Quantitative Chromosome Conformation Capture            Raffaella Nativio, Yoko Ito, and Adele Murrell 12. Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing            Sébastien A. Smallwood and Gavin Kelsey Part V: Analysis of Imprinted Expression 13. Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies            Sarah Rose Huffman, Md Almamun, and Rocío Melissa Rivera 14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs            Mathieu Rederstorff 15. Co-Immunoprecipitation of Long Non-Coding RNAs            Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil Part VI: Imprinting in Plants 16. Specialized Technologies for Epigenetics in Plants            Wenyan Xiao Part VII: Evolution of Imprinted Genes 17. Computational Studies of Imprinted Genes            Martina Paulsen 18. Insights on Imprinting from Beyond Mice and Men            Andrew Pask 19. Non-Mammalian Parent-Of-Origin Effects            Elena de la Casa-Esperón 14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs            Mathieu Rederstorff 15. Co-Immunoprecipitation of Long Non-Coding RNAs            Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil Part VI: Imprinting in Plants 16. Specialized Technologies for Epigenetics in Plants            Wenyan Xiao Part VII: Evolution of Imprinted Genes 17. Computational Studies of Imprinted Genes            Martina Paul

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